ROC Cell Cultivation System

Description:

Improved micro­scopes and dif­fer­ent opti­cal tech­niques now allow high stan­dards in the micro­scop­ic analy­sis of liv­ing cells. Heat­ed micros­cope stages and cli­mate boxes offer the pos­si­bil­i­ty of observ­ing cells and tis­sues under sta­bi­lized tem­per­a­tures. Dif­fer­ent cli­mate boxes are capa­ble of hold­ing the desired tem­per­a­ture and the pH value of the medi­um in which the cells are being cul­tured by a reg­u­la­tion of CO₂. In addi­tion, the O₂ value can be reg­u­lat­ed under con­di­tions like in vivo.

The ROC Cham­ber Sys­tem meets the demands of dif­fer­ent micro­scop­ic meth­ods in the obser­va­tion and analy­sis of liv­ing cells. It allows both Open and Closed Cul­ti­va­tion and Open Perfusion.

The inserts for Open or Closed Cul­ti­va­tion or for Per­fu­sion are fixed onto a base plate. Open and Closed Cul­ti­va­tion as well as Open Per­fu­sion with 2 input/output canals (includ­ed in the Set) are pos­si­ble. The Open Cul­ti­va­tion Setup allows a rapid entrance to the cells and an easy medi­um exchange.

The ROC Cell Cul­ti­va­tion Sys­tem has a round base­plate and an obser­va­tion area of Ø 29 mm. The vol­ume of the cham­ber is 0.66 - 1.32 ml (closed) and up to 2.5 ml (open). The cells are cul­ti­vat­ed on cov­er­slips with a thick­ness of 0.17 mm (Ø 42 mm).

• Sys­tem for all micros­cope techniques
• Suit­able for short- and long-term cultivation
• Open and closed cul­ti­va­tion as well as open per­fu­sion are pos­si­ble (for open per­fu­sion, an expan­sion set has to be ordered separately)
• In the case of open cul­ti­va­tion, the cham­ber can be pro­tect­ed from evap­o­ra­tion by a Foil­Cov­er
• In all ROC appli­ca­tions, the cells can be cul­ti­vat­ed on glass
• A pre-cultivation of cells on cover glass­es (Schott: 170 µm ± 5 µm, No. 1.5) in Petri dish­es is pos­si­ble; the assem­bling of the ster­ile ROC cham­ber occurs in a lam­i­nar air flow
• The ROC cham­ber can be heat­ed with­out addi­tion­al adapter in the Heat­ing Frames MH 2000 Series, KH 2000 Series and AH 2000 Series, for the use in com­bi­na­tion with the Heat­ing Insert P 2000, a spe­cif­ic adapter (# 0728.002) is required
• All parts of the ROC cham­ber can be ster­il­ized by auto­clav­ing (121°C) or by dry heat­ing (165-170°C)

For a bet­ter cell cul­ti­va­tion, the glass­es can be ster­ile­ly coat­ed with Col­la­gen IV, Poly-L-Lysine, Poly-D-Lysine, Fibronectin or others.

For longer obser­va­tions of cell and tis­sue cul­tures under the invert­ed micros­cope, the ROC cham­ber should be placed into a Heat­ing Frame 2000 or Heat­ing Insert P 2000 by PeCon.

Overview ROC System: